Natural Competence for Transformation in Lactobacili

Authors

• Michael V. Tyurin CEO, Executive Chairman, President, Microbial Biocatalyst International, Inc. and Inorgcarbdiesel, Inc. Houston, TX 77230

Abstract

We have detected the changes in the molecular biologic properties of the lascobacilus strain Lactibacillus buchneri NRRLB1837 after its storage in the lyophilized state using some patented lyophilization media.  The change noticed was the 3 orders of magnitude decrease of the natural competence for the genetic transformation capability of said lac-tobacillus strain.  Using noted difference we have transformed L. buchneri NRRLB1837 with the largest plasmid of Lactobacillus casei MT205, possessing strong antibacterial activity against gram-positive, gram-negative bacteria and Candida albicans.  The trans formants became resistant to Erythromycin (30 mcg/ml) and also had strong antibacterial activity, just like L. casei MT205, which was extractable from the concentrated by the evaporation cultural fluid of the respective transformants, The role of the lyophilization medium on the molecular biological properties of the lactobacilli stored lyophilized is dis-cussed